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SERANA热灭活胎牛血清FBS-HT500

货号:FBS-HT500

规格:500ml/100ml

产品名称: Heat-Inactivated  Fetal Bovine Serum 

目录价:5900元/询价


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产品编号 SERANA FBS-HT500
  • DETAILS
  • Overview

【产品介绍】

SERANA热灭活目的是祛除血清中补体等对热敏感的物质。激活的补体参与溶解细胞事件,刺激平滑肌收缩,细胞和血小板释放组胺,激活淋巴细胞和巨噬细胞。本产品采自新西兰源健康母牛的出生前胎牛,热灭活是在56℃水浴中严格控温30分钟,可以灭活补体系统,可以节省特殊用途客户时间,不用再次灭活,直接解冻后即用。该款胎牛血清是一款性能强劲的AUS优选产品。

经过了热灭活处理,有效减少了血清中的有害的外源因子,同时使得血清中的补体成分灭活,对于一些培养条件要求苛刻特殊的细胞,比如免疫学研究细胞培养,ES细胞、昆虫细胞和平滑肌细胞比较适用。


  • 三次0.1um无菌过滤

  • 支原体检测和病毒筛查

  • 干冰运输,建议储存温度为-20°c

 

胎牛血清作用

1.SERANA提供对维持细胞生长的稳定,基础培养基中没有或量很少的营养物主要的低分子营养物。

2.SERANA提供结合蛋白,能识别维生素、脂类、金属和其他激素等,能结合或调变它们所结合的物质活力。

3.特定情况下结合蛋白质能与有毒金属和热原质结合,起到解毒作用。

4.是细胞贴壁、铺展在塑料培养基质上所需要的因子来源。

5.SERANA FBS起酸碱度缓冲液作用。

6.蛋白酶抑制剂,使在细胞传代时使剩余胰蛋白酶失活,保护细胞不受损伤

 

储存条件和有效期

储存温度:-10℃~-20℃

 

科研使用,不用于临床诊断和治疗。


Overview

SERANA has deep screening experience producing serum of the highest standard. In order to obtain fetal bovine serum with high-level parameters, serana adopts a very strict quality control system. It has been widely used in the cell culture process of many universities, research institutes and R&D companies in China, and the product quality has also won good word of mouth.

 

This fetal bovine serum is a powerful serum product suitable for the cultivation of delicate cell lines. Using 3 times of 0.1μm filtration method and quality control testing, after cell culture testing, tumor cells, lymphocytes, hybridomas, endothelial cells, primary cells, etc. can use this product.

 

SERANA Fetal Bovine Serum delivers outstanding value for basic cell culture, specialized research and specific assays, earning researchers' trust with consistent quality and award-winning support to help meet your research needs and budget requirements‎

Serum is the blood component that can be obtained after coagulation and by removing cellular components. Besides  serum proteins it contains, e.g. growth factors, amino acids and hormones. This comprehensive mix makes serum  one of the most important supplements, supporting cells to grow and proliferate in vitro cell culture. Of special interest is the fetal bovine serum (FBS). It is especially rich in growth factors and is particularly low in  antibodies, which may influence the cell culture work.

Shelf life5 years

Sera Category: Premium (Secure)

Origin: china/New Zealand/AUS

Endotoxin level: <=5 EU/mL.

Hemoglobin level: <=30 mg/dL (levels routinely <=25 mg/dL)

 

Thawing

Remove serum from -10°C (or lower) storage and place in a refrigerator overnight at 2°C to 8°C. Transfer the serum to a 37°C water bath, agitate periodically to mix the solutes concentrated at the bottom of the container. Do not hold the serum at 37°C any longer than necessary after thawing. Thawing serum in a bath above 40°C without mixing can denature the concentrated proteins in the bottom of the container and precipitates might form in the bottle. Thawing serum at higher temperatures is not recommended. Alternatively, bottles can be placed directly from -10°C (or lower) storage into a 37°C water bath. Bottles should be agitated to enhance mixing and thawing. Turbidity and flocculent material might be present after thawing or after prolonged storage.  Experience indicates that regardless of the method used to thaw serum, it is critical that it is mixed during the thawing process to prevent the formation of gradients and subsequent precipitation. Because of differences in thawing rates of different components, serum will form a gradient if it is not mixed as it thaws. If serum is allowed to remain in such a gradient state, precipitation is likely to occur. Handle bottles that have been stored in freezer carefully. Avoid large temperature shifts and protect the serum from exposure to light. Wear protective clothes and equipment. Storage requirements are listed on the product label

Only sera batches which pass our strict quality control are released for sale. Standard parameters which are  determined include pH, osmolality, content of protein, albumin, IgG and hemoglobin, endotoxin level, sterility,  mycoplasma detection and virus testing.

 

Serum quality testing 

 

Product code   

FBS-AS

FBS-UE

FBS-ESA

FBS-DU

FBS-DT

FBS-SG

FBS-HT

Filtration      

Triple100 nm

Triple 100 nm

Triple 100 nm

Triple 100 nm

Triple 100 nm

Triple 100 nm

Triple 100 nm

Endotoxin (EU/mL)      

≤ 20

≤ 50

≤ 25

≤ 10

≤ 10

≤ 25

≤ 25

Osmolality (mOsm/kg).

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Total protein (mg/dL)

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Sterility (bacteria and fungi)

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Fluorescent antibody

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Cytopathic agents

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Hemadsorbing agents

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Mycoplasma

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Protein testing

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Trace metals/iron

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Vitamins, hormones

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Electrophoretic profile

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IgG (mg/mL)

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General culture recommendations

Supplementation of classical media such as Dulbecco’s Modified Eagle’s Medium (DMEM) is recommended at a range between 5% and 10% FBS to support culture of a wide variety of cell lines and applications

Bovine Serum of Chinese and New Zealand/Australia origin

In addition to the characteristic fetal bovine serum originating in China, we also provide

A range of bovine serum from New Zealand and Australia. New serum products are carefully collected, processed and filtered to provide high safety for cattle

disease.

Extensive quality testing is carried out to ensure

Consistency and performance of SERANA serum products.

Each batch, depending on the serum used.

 

Precautions and Disclaimer

This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards  and safe handling practices.

Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.

 


FREQUENTLY ASKED QUESTIONS (FAQS)
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Q: Will it damage some factors in Serana serum if it is inactivated?

Answer: Heating can inactivate the complement system. Activated complement is involved in lytic events, stimulates smooth muscle contraction, releases histamine from cells and platelets, and activates lymphocytes and macrophages. Heat-inactivated serum is recommended for immunological studies, culturing ES cells, insect cells, and smooth muscle cells. Inactivation generally does not damage some factors in serum.

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