Overview
SERANA has deep screening experience producing serum of the highest standard. In order to obtain fetal bovine serum with high-level parameters, serana adopts a very strict quality control system. It has been widely used in the cell culture process of many universities, research institutes and R&D companies in China, and the product quality has also won good word of mouth.
This fetal bovine serum is a powerful serum product suitable for the cultivation of delicate cell lines. Using 3 times of 0.1μm filtration method and quality control testing, after cell culture testing, tumor cells, lymphocytes, hybridomas, endothelial cells, primary cells, etc. can use this product.
SERANA Fetal Bovine Serum delivers outstanding value for basic cell culture, specialized research and specific assays, earning researchers' trust with consistent quality and award-winning support to help meet your research needs and budget requirements
Serum is the blood component that can be obtained after coagulation and by removing cellular components. Besides serum proteins it contains, e.g. growth factors, amino acids and hormones. This comprehensive mix makes serum one of the most important supplements, supporting cells to grow and proliferate in vitro cell culture. Of special interest is the fetal bovine serum (FBS). It is especially rich in growth factors and is particularly low in antibodies, which may influence the cell culture work.
Shelf life:5 years
Sera Category: Premium (Secure)
Origin: china/New Zealand/AUS
Endotoxin level: <=5 EU/mL.
Hemoglobin level: <=30 mg/dL (levels routinely <=25 mg/dL)
Thawing
Remove serum from -10°C (or lower) storage and place in a refrigerator overnight at 2°C to 8°C. Transfer the serum to a 37°C water bath, agitate periodically to mix the solutes concentrated at the bottom of the container. Do not hold the serum at 37°C any longer than necessary after thawing. Thawing serum in a bath above 40°C without mixing can denature the concentrated proteins in the bottom of the container and precipitates might form in the bottle. Thawing serum at higher temperatures is not recommended. Alternatively, bottles can be placed directly from -10°C (or lower) storage into a 37°C water bath. Bottles should be agitated to enhance mixing and thawing. Turbidity and flocculent material might be present after thawing or after prolonged storage. Experience indicates that regardless of the method used to thaw serum, it is critical that it is mixed during the thawing process to prevent the formation of gradients and subsequent precipitation. Because of differences in thawing rates of different components, serum will form a gradient if it is not mixed as it thaws. If serum is allowed to remain in such a gradient state, precipitation is likely to occur. Handle bottles that have been stored in freezer carefully. Avoid large temperature shifts and protect the serum from exposure to light. Wear protective clothes and equipment. Storage requirements are listed on the product label
Only sera batches which pass our strict quality control are released for sale. Standard parameters which are determined include pH, osmolality, content of protein, albumin, IgG and hemoglobin, endotoxin level, sterility, mycoplasma detection and virus testing.
Serum quality testing
Product code | FBS-AS | FBS-UE | FBS-ESA | FBS-DU | FBS-DT | FBS-SG | FBS-HT |
Filtration | Triple100 nm | Triple 100 nm | Triple 100 nm | Triple 100 nm | Triple 100 nm | Triple 100 nm | Triple 100 nm |
Endotoxin (EU/mL) | ≤ 20 | ≤ 50 | ≤ 25 | ≤ 10 | ≤ 10 | ≤ 25 | ≤ 25 |
Osmolality (mOsm/kg). | · | · | · | · | · | · | · |
Total protein (mg/dL) | · | · | · | · | · | · | · |
Sterility (bacteria and fungi) | · | · | · | · | · | · | · |
Fluorescent antibody | · | · | · | · | · | · | · |
Cytopathic agents | · | · | · | · | · | · | · |
Hemadsorbing agents | · | · | · | · | · | · | · |
Mycoplasma | · | · | · | · | · | · | · |
Protein testing | · | · | · | · | · | · | · |
Trace metals/iron | · | · | · | · | · | · | · |
Vitamins, hormones | · | · | · | · | · | · | · |
Electrophoretic profile | · | · | · | · | · | · | · |
IgG (mg/mL) | · | · | · | · | · | · | · |
General culture recommendations
Supplementation of classical media such as Dulbecco’s Modified Eagle’s Medium (DMEM) is recommended at a range between 5% and 10% FBS to support culture of a wide variety of cell lines and applications
Bovine Serum of Chinese and New Zealand/Australia origin
In addition to the characteristic fetal bovine serum originating in China, we also provide
A range of bovine serum from New Zealand and Australia. New serum products are carefully collected, processed and filtered to provide high safety for cattle
disease.
Extensive quality testing is carried out to ensure
Consistency and performance of SERANA serum products.
Each batch, depending on the serum used.
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.